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CRISPR 質(zhì)粒
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13
- Zhou J, Yin LJ, Dong YA, et al. CRISPR-Cas13a based bacterial detection platform: Sensing pathogen Staphylococcus aureus in food samples. Analytica Chimica Acta. 2020.
- doi: 10.1016/j.aca.2020.06.041
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14
- Nan YL, Ouyang LM, Chu J. In vitro CRISPR/Cas9 system for genome editing of Aspergillus niger based on removable bidirectional selection marker AmdS. Biotechnol Appl Biochem. 2020.
- doi: 10.1002/bab.1996
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15
- Wang BK, Li N, Huang SY, et al. Enhanced soluble sugar content in tomato fruit using CRISPR/Cas9-mediated SlINVINH1 and SlVPE5 gene editing. PeerJ. 2021.
- doi: 10.7717/peerj.12478
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16
- Tan G, Lin CJ, Huang CY, et al. Radiosensitivity of colorectal cancer and radiation-induced gut damages are regulated by gasdermin E. Cancer Lett. 2022.
- doi: 10.1016/j.canlet.2021.12.034
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17
- Cao CC, Ma Q, Huang XB, et al. Targeted Demethylation of the PLOD2 mRNA Inhibits the Proliferation and Migration of Renal Cell Carcinoma.Frontiers in Molecular Biosciences, 2020.
- doi: 10.3389/fmolb.2021.675683
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18
- Dong T, Zhang SY, Chang Y, et al. The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system. Stem Cell Research. 2021.
- doi: 10.1016/j.scr.2021.102196
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19
- Zhou YY, Lin L, Wang H, et al. Development of a CRISPR/Cas9n-based tool for metabolic engineering of Pseudomonas putida for ferulic acid-to-polyhydroxyalkanoate bioconversion. Communications Biology. 2020.
- doi: 10.1038/s42003-020-0824-5
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20
- Wu FJ, Li Xw, Bai R, et al. Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system. Stem Cell Research. 2020.
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21
- Triozzi P, Schmidt HW, Dervinis C, et al. Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba. Tree Physiology. 2021.
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22
- Yankun Y, Guoqiang L, Xiao C,et al. High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris.Enzyme and Microbial Technology, Volume 138, August 2020.
- doi:10.1016/j.enzmictec.2020.109556
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23
- Zhu M , Sun L , Lu X , et al. Establishment of a transient CRISPR-Cas9 genome editing system in Candida glycerinogenes for co-production of ethanol and xylonic acid[J]. Journal of Bioscience and Bioengineering, 2019.
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24
- Li J, Chen Z, Chen F, et al. Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein[J]. bioRxiv, 2019: 614859.
- doi:10.1101/614859
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